Application

ELISA (1:2000-1:5000)r>Flow Cytometry (1:50-1:200)r>Immunohistochemistry (1:50-1:200)

General description

Streptavidin conjugated to TEXAS RED^® fluorescent dye. Specific for biotin, biotinylated proteins, or biotinylated antibodies.

Legal Information

Texas Red is a registered trademark of Life Technologies

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Biotin activity, absorbance ratio, and label:protein molar ratio are determined on a lot-specific basis. Use 1 to 3 µg of the conjugate per 1 x 10⁶ cells suspended in a volume of 0.1 ml. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

1 ml in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM bicarbonate-borate buffer, 0.9% NaCl, 5 mg/ml BSA, pH 8.1.

Reconstitution

Do not dilute the entire sample at one time. Withdraw aliquots as needed with a micropipette and dilute with 1% solution of BSA in 50 mM sodium phosphate buffer, pH 7.4 and 0.05% sodium azide.

Warning

Toxicity: Standard Handling (A)